Composite

Part:BBa_K3796207:Design

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)


Patp2-rbs-gfp-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a short sequence CCTGCAGG before bjGFP to ensure proper distance between rbs and bjGFP, leading to higher translation efficiency.


Source

The promoter Patp2 comes from Corynebacterium glutamicum ATCC 13032.

The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic.

The bjGFP is originally chemical syhthesized and we amplified this sequence from the plasmid pRJPaph-bjGFP.

The rrnB terminators sequence information is from BBa_K3568009 and the sequences for rrnB T1 and T2 are carried by the plasmid pXMJ19.


References